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Reduced levels of a regulatory DC subtype may be linked to the development of spontaneous autoimmune disease in aging CD48 deficient mice
Author(s) -
TAMIR AYALA,
LATCHMAN YVETTE
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.411
Subject(s) - immune system , biology , cd8 , autoimmune disease , immunology , antibody , il 2 receptor , t cell
CD48 is a member of the CD2 superfamily of co‐stimulatory molecules and has an important role in immune response regulation. Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease. Its genetic basis indicates that three regions on different chromosomes (SLE1, 2 and 3) are linked with susceptibility to the disease at different severities. A sub‐region of SLE1 on chromosome one (SLE1b) includes members of the CD2 superfamily. We have previously found that aging CD48 deficient ( −/− ) C57BL/6 mice develop spontaneous autoimmune disease, indicated in elevated levels of dsDNA, IgG and IgM antibodies and of activation markers on CD4 + and CD8 + T cells. We found no difference in the levels of CD4 + CD25 + T cells in both groups of mice. However, levels of CD11c low CD45rb high DCs, recently characterized as a regulatory DC subtype (rDC), were at least 2‐times lower in the bone marrow and the spleens of CD48 −/− compared to WT mice. After LPS stimulation, expression of costimulatory molecules, CD40 and DEC205 were upregulated on CD48 −/− rDC in contrast to WT rDC where the expression remained low. In addition, OT‐II‐ derived CD4 + T cell proliferated and produced higher levels of IFN‐γ and IL‐4 when cultured with OVA‐pulsed CD48 −/− rDC compared to those cultured with OVA‐pulsed WT rDCs. Our results indicate that low levels of rDC may be responsible for impaired regulation of the immune response in CD48 −/− mice.