Premium
TISSUE‐SPECIFIC EXPRESSION OF B‐CELL TRANSLOCATION GENE 2 (BTG2) AND ITS FUNCTION IN T‐CELL IMMUNE RESPONSES IN A TRANSGENIC MOUSE MODEL
Author(s) -
Terra Rafik,
Luo Hongyu,
Qiao Xiaoying,
Wu Jiangping
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.401
Subject(s) - biology , immune system , genetically modified mouse , t cell , transgene , in vivo , b cell , endocrinology , medicine , immunology , gene , antibody , biochemistry , microbiology and biotechnology
BTG2 belongs to the anti‐proliferative (APRO) gene family. According to previous in vitro studies, BTG2 overexpression leads to delayed cell cycling. We investigated BTG2 expression during mouse ontogeny and its immune and circadian functions in this study. In situ hybridization showed that BTG2 was expressed at high levels in the central nervous system, liver, stomach, thymus, spleen, skin, adrenal gland, pituitary gland and salivary glands during embryonic (E10 to E17), postnatal (P1 and P10) and adult stages. Expression was observed in organs and tissues from adult mice with and without a robust proliferation program. Thus, the gene might have important functions that are both related and unrelated to proliferation. BTG2 expression was induced after in vitro T‐cell receptor stimulation in T cells using anti‐CD3 Abs. However, transgenic (Tg) mice with actin promoter‐driven expression of BTG2 showed normal in vitro and in vivo T‐cell responses, such as thymus development, T‐cell activation marker expression, T‐cell proliferation and migration, as well as in vivo delayed type hypersensitivity reactions. Although BTG2 was expressed in the suprachiasmatic nucleus and pineal gland in the brain, BTG2 Tg mice had no abnormal circadian behavior. Our data on BTG2 expression during ontogeny provide useful clues for the further investigation of BTG2 function. Additional studies are warranted to examine its role in immune and other systems.