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Tc17, a new subset of CD8 T cells
Author(s) -
Hamada Hiromasa,
Reome Joyce B.,
Misra Sara K.,
McKinstry Kai K.,
Strutt Tara M.,
Swain Susan L.,
Dutton Richard W.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.358
Subject(s) - perforin , cytotoxic t cell , cd8 , granzyme b , immunology , gata3 , biology , antibody , in vitro , immune system , transcription factor , biochemistry , gene
IL‐17 producing CD4 + (Th17) and CD8 + (Tc17) effector cells are found in the lung following both primary and secondary challenge with influenza A. Tc17 effectors can be generated in vitro by four day culture of naïve CD8 T cells from OT‐1 TcR transgenic mice in the presence of a cocktail of cytokines including IL‐1β, IL‐2, IL‐6, TGF‐β, IL‐21 and IL‐23 and antibodies to IFN‐γ and IL‐4. The naïve T cell numbers expand 10∼20 fold in culture and a majority of the resulting cells secrete IL‐17, TNF‐α and IL‐2 upon restimulation but very few cells that secrete IFN‐γ or are positive for granzyme B, in marked contrast to Tc1. Tc17 populations express message for IL‐17, IL‐17F and IL‐22 and smaller amounts for IL‐10 and TGF‐β̃ They do not express message for perforin and exhibit no cytolytic activity. Populations of Tc17 cells express message for RORγt and FoxP3, but are negative for T‐bet and GATA3. Populations of both Tc1 and Tc17 effectors can protect naïve recipients against lethal influenza A infection. This work was supported by NIH AI046530.