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Identification of hDUSP6 Enhancer Which Is Involved in Supporting Gene Expression in Embryonic Stem Cells
Author(s) -
Zhang Jiaxing,
Maruyama Masayoshi,
Nishimoto Masazumi,
Okuda Akihiko
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.31
Subject(s) - embryonic stem cell , dusp6 , enhancer , biology , gene expression , gene , regulation of gene expression , stem cell , cellular differentiation , microbiology and biotechnology , genetics , protein phosphatase 2 , protein subunit
To identify genes involved in maintaining stem cell function of embryonic stem cells(ESCs), we compared gene expression profilesbetween undifferentiated and differentiated ESCs using DNA Microarrays. As expected, we identified hundreds of genes whose expression is rather specific in undifferentiated ESCs including Oct‐3/4. Among them, we focused on dual specificity phosphatase 6 (DUSP6) because of two reasons. One is that the gene has been shown to have undifferentiated state‐specific expression also in human ESCs. The second reason is that DUSP6 is one of negative regulators of MAP kinase which is involved in differentiation of ESCs. Therefore, we set out to investigate the role of DUSP6 in ESCs and elucidate the molecular basis of specific expression of the DUSP6 gene in undiffenretiated ESCs. Here, we demonstrate the identification of a regulatory element which appears to be involved in DUSP6 gene in ESCs. The element has no binding site for Oct‐3/4 and no similarity to any regulatory regions which have been reported to be involved in expression in ESCs.