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Highly efficient neural differentiation of peripheral blood human hematopoietic stem cells
Author(s) -
Voelkel Tobias,
Widera Darius,
Dittmar Kurt E.J.,
Moebius Jeannette M,
Kaltschmidt Christian,
Kaltschmidt Barbara,
Piechaczek Christoph
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.30
Subject(s) - neurosphere , neural stem cell , stem cell , haematopoiesis , biology , adult stem cell , microbiology and biotechnology , embryonic stem cell , neuroepithelial cell , nestin , neuroscience , immunology , biochemistry , gene
Neural stem cells are a potential source of cells for cell therapy of neurodegenerative diseases or drug screening. Ethical and practical considerations limit the application of neural stem cells derived from human embryonic stem cells or adult brain tissue. Therefore, alternative sources of adult human neural stem cells are of high interest for both, ‐ basic research and potential clinical use as well. These sources have to satisfy the claims of readily accession, rapid expansion in serum‐free media and reliably induction to a neural fate. Hematopoietic stem cells (HSCs) isolated from mobilized leukapheresis were cultured and expanded in chemically defined, serum‐free media. The culture resulted in aggregation to neurosphere‐like clusters and large numbers of CD133‐, nestin+ and Sox‐2+ neural‐like stem cells. Neural stem cells derived from HSCs (hNSCs) are able to migrate in response to chemotactic stimuli and can be differentiated into mature neurons (bIII‐tubulin+ and NF+) and astrocytes (GFAP+). Differentiated neurons show functional characteristics such as voltage‐sensitive calcium transients. Our novel method provide nearly limitless numbers of neural stem cells from an easily accessible autologous adult human source, which could be used as a starting point for further experimental studies and potential therapeutic use.