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5‐Methylsophoraflavanone B inhibits adipocyte differentiation through an activation of GATA‐2 transcription factor
Author(s) -
Nho Chu Won,
Kim Chul Young,
Lee Hee Ju,
Selenge Dangaa,
Lee Saet Byoul,
Kang Kyungsu
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.272
Subject(s) - adipocyte , transcription factor , oil red o , adipogenesis , microbiology and biotechnology , 3t3 l1 , chemistry , cellular differentiation , peroxisome proliferator activated receptor , receptor , biology , biochemistry , adipose tissue , gene
The transcription factor GATA‐2, a key regulator of adipocyte differentiation, is expressed in adipocyte precursors, and directly suppresses peroxisome proliferator‐activated receptor γ (PPAR γ). Constitutive expression of GATA‐2 is known to hold the cells in undifferentiated state. Hence, GATA‐2 activators could be good candidates for anti‐obesity agents. A prenyl flavanone, 5‐methylsophoraflavanone B (5‐MSF) was isolated from a Mongolian medicinal plant Sophora flavescens Soland. To evaluate the inhibitory activity of 5‐MSF on adipocyte differentiation, we induced differentiation of 3T3‐L1 cells in the presence of 5‐MSF. 5‐MSF evidently inhibited adipocyte differentiation at 100 μM, which was confirmed by a microscopic observation and a quantification of lipid droplets after Oil Red O staining. Further molecular study revealed that 100 μM of 5‐MSF slightly increased the protein expression of GATA‐2 over the vehicle control in undifferentiated 3T3‐L1 cells, while it decreased the protein expression of PPAR γ in mature 3T3‐L1 cells concurrently. In conclusion, 5‐MSF inhibited adipocyte differentiation through the activation of the GATA‐2, and therefore it could be developed as a potential anti‐obesity agent.