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Functional analyses of human nucleolar protein, Nucleophosmin
Author(s) -
Amin Mohammed Abdullahel,
Matsunaga Sachihiro,
Fukui Kiichi
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.267
Subject(s) - nucleophosmin , microbiology and biotechnology , mitosis , centrosome , biology , cell cycle , chromatin , hela , cell culture , computational biology , cell , genetics , gene
Nucleophosmin (NPM) is an abundantly and ubiquitously expressed nucleolar pohosphoprotein, which plays a key role in the regulation of a number of processes including ribosome biosynthesis and maintenance of genomic integrity. Although several other functions of NPM such as centrosome duplication and chromatin regulation have been reported, its roles during the cell cycle are still unknown. In this study, we have shown the very clear dynamic localization of NPM throughout the cell cycle of HeLa cell by paraformaldehyde (PFA) fixation and immunofluorescence microscopy. Using a combination of high‐resolution microscopy and RNAi method, we have found important roles of NPM in cellular growth and mitotic process in HeLa cell. This work was supported by the grant for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.