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YJL185c encodes a peroxisomal protein that binds Inp1p and is required for peroxisome retention
Author(s) -
Fagarasanu Monica Eliza,
Mast Fred,
Rachubinski Richard
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.263
Subject(s) - peroxisome , organelle , microbiology and biotechnology , saccharomyces cerevisiae , biology , protein targeting , cell division , compartmentalization (fire protection) , green fluorescent protein , yeast , biochemistry , gene , cell , membrane protein , enzyme , membrane
The inheritance of cellular organelles from mother cell to daughter cell is critical for eukaryotic cells to maintain the metabolic benefits of compartmentalization. Because of its asymetrical division, the budding yeast Saccharomyces cerevisiae has been used extensively to understand the molecular pathways involved in the inheritance of different organelles. The inheritance of peroxisomes is dependent on the peroxisomal protein Inp1p, which is directly involved in tethering peroxisomes to anchoring structures at the cell cortex. Although Inp1p is a major factor acting in the peroxisomal retention pathway, we proposed that its action in peroxisome retention is regulated by additional proteins. with which it interacts. A protein of unknown function encoded by the open reading frame YJL185c was shown to interact with Inp1p by the yeast two‐hybrid system. Cells deleted for YJL185c exhibit an altered peroxisome morphology and a preferred localization of peroxisomes to the bud neck. Yjl185p tagged with green fluorescent protein, GFP, shows both a peroxisomal and a weak cortical localization. Our studies help to clarify the mechanism of action of Inp1p in peroxisome inheritance and identify Yjl185p as a novel protein involved in peroxisome inheritance.