Premium
CRP3/MLP Expression is Induced Human Saphenous Vein Graft Arterialization
Author(s) -
Campos Luciene Cristina Gastalho,
Miyakawa Ayumi Aurea,
De Oliveira Dallan Luis Alberto,
Krieger José Eduardo
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.242
Subject(s) - ex vivo , vein , gene expression , smooth muscle , immunohistochemistry , in vivo , artery , vascular smooth muscle , anatomy , medicine , biology , endocrinology , chemistry , gene , biochemistry , microbiology and biotechnology
Cysteine‐rich proteins (CRPs) are present in the cytoskeleton and expressed during cardiovascular development. Recently CRP proteins have also been demonstrated to act as potent transcriptional cofactors involved in the late stage of smooth muscle cells (SMCs) differentiation. Presently, we wanted to investigate the expression of CRPs (CRP1, CRP2 and CRP3/MLP) in human arteries (mammary artery, MA) and veins (saphenous vein, SV). Using RT‐PCR, we observed similar levels of CRP1 and CRP2 in both MA and SV segments. In contrast, the expression of CRP3 was 4 times higher in MA compared to SV (MA: 1.25±0.11 vs. SV: 0.30±0.14, N=5, p<0.01). Moreover, when we examined SMCs primary cultures from MA and SV, we found that CRP3 was expressed only in SMCs from MA (0.86±0.09 vs. SVSMC: 0, N=5, p<0.001, respectively). Interestingly, upon SV arterialization using an ex vivo flow through system (50mL/min, 80mmHg, for 24h), we observed a 7 fold induction in CRP3 expression. Collectively, our data suggest that CRP3 gene is expressed mainly in SMCs from MA and can be induced in SV submitted to arterialization, which is consistent with the idea that CRP3 is an arterial SMC phenotypic marker.