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18F‐2, Fluoro 2, deoxy‐glucose (FDG) uptake in Wistar rat heart and brain after chronic dietary carbohydrate (CHO) restriction
Author(s) -
Fine Eugene J,
Miao Weibing,
Koba Wade,
Blaufox M Donald
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.236
Subject(s) - ketone bodies , insulin , medicine , endocrinology , glucose uptake , glucose transporter , positron emission tomography , transporter , carbohydrate metabolism , nuclear medicine , chemistry , metabolism , biochemistry , gene
Cardiac FDG uptake varies in human positron emission tomography (PET) despite efforts to standardize protocols, a fact of physiologic interest since heart metabolizes fatty acids, ketone bodies (KB) as well as glucose (Gl). Brain Gl uptake uses insulin‐independent transporters. Objective: To test in a rodent model that chronic very low CHO (VLC) diets result in lower FDG heart, but not brain uptake compared with high and intermediate CHO (HC & IC) diets. Methods: 15 Wistar rats (age 6–8 wks) were randomized to 3 diet groups (n= 5) of LC (0.1% of total energy), IC (52%) and HC (78%) content (3), keeping protein and total energy constant. After 4 wks, blood for KB, Gl, insulin and glucagon was stored for analysis, followed by small animal PET within 2 days. Each rat received 600 μCi FDG by tail vein, followed in 1 hr by whole body PET. Maximum standardized uptake values (SUVmax) were determined. Mean SUVmax (MSUV) data were compared using ANOVA among all comparisons. Results: Heart MSUV was 3.2±1.2 (LC); 10.6±2.5 (IC); 10.6±2.8(HC); p<0.0001 for LC but p = NS for IC vs. HC. Brain values MSUV = 4.2±0.7 (LC), 3.9±0.6 (IC), 3.5±0.7 (HC) did not differ with diet (p = NS). Conclusions: This prospective study demonstrates reduced cardiac uptake but stable brain uptake of FDG in chronic VLC diets, consistent with differing insulin sensitivity of GL transporters in these organs.