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Effects of different fluid replacements following dehydration on oxidative DNA damage during rest and exercise
Author(s) -
Paik IlYoung,
Jeong MyungHyun,
Kim YoungIl,
Jin HwaEun,
Cho SuYoun,
Roh HeeTae,
Shi Xiaocai
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.179
Subject(s) - dehydration , malondialdehyde , chemistry , zoology , dna damage , body fluid , comet assay , antioxidant , medicine , biochemistry , biology , dna
To investigate the effects of different types of oral rehydration with either water or sports drink (Gatorade) on biochemical responses, DNA damage and exercise performance. Ten healthy subjects randomly went through four different trials: Control (C), 3% Dehydration (D), 3% Dehydration followed by rehydration with water (W) or sports drink (S). Prior to exercise, the groups D, W and S were dehydrated by sauna to lose 3% body weight, and then groups W and S were allowed to drink either water or sports drink for a 100% rehydration. After 2h rehydration at rest, subjects started to exercise on a treadmill at 80%VO 2max till exhaustion. Lymphocyte DNA damage was detected by the Comet assay. Exercise time to exhaustion was significantly longer for groups C and S comparing with group D ( p <.05). There were no significant differences among groups for lactate and creatine kinase (CK) concentration at each time point during the experiment. Malondialdehyde (MDA) significantly increased during dehydration and decreased during rehydration for groups W and S ( p <.05). All groups showed a continuous increase of MDA during exercise and a significant decrease at 60 min of recovery ( p <.05). Dehydration and exercise significantly increased and rehydration significantly decreased total antioxidant status (TAS) ( p <.05). Tail moment, the best indications of DNA damage, was significantly lower at 60 min of recovery in group S than group D ( p <.05). These results suggest that dehydration impairs performance and tends to produce more DNA damage. Restoration of body fluid using water or a sports drink attenuates the effect of dehydration on DNA damage, but rehydration with a sports drink prolongs the exercise time to exhaustion during high intensity, less than one hour exercise.