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Bicarbonate‐regulated soluble Adenylyl Cyclase (sAC) mRNA expression in peripheral and central chemoreceptors
Author(s) -
Nunes Ana Rita Silva Martins,
Monteiro Emilia C,
Johnson Shereé M,
Gauda Estelle B.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.171
Subject(s) - endocrinology , medicine , biology
Peripheral and central chemoreceptors in the carotid body (CB) and brainstem (BS), respectively are modulated by pH/HCO 3 − via cAMP mechanisms. HCO 3 increases sAC activity in germ cells from testis (Mol Reprod Dev.2000; 566−11). We hypothesized that sAC mRNA levels would be greater in the CB and BS than in non chemosensitive tissues. Tissue from Sprague‐Dawley rats at postnatal day 16 (n=6 animals per experiment) were used to compare sAC mRNA levels by qRT‐PCR using primers complementary to rat sAC and GAPDH (housekeeping gene) analyzed with the Pfaffl method. sAC mRNA expression in the CB was compared to that of chemosensitive [raphe nucleus (RN), ventrolateral medulla (VLM) and locus coeruleus (LC)], and non chemosensitive [cortex, testis, superior cervical (SCG), petrosal (PG) and nodose ganglia (NG)] tissues. RESULTS: Other than the testis, sAC mRNA expression was between 2–11% of CB expression in other tissues (TABLE, p<0.01 vs CB). Greater sAC mRNA expression in the CB compared to central chemo and non‐chemosenstive tissues suggest that sAC could contribute to the regulation of cAMP levels involved in chemosensitivity in the CB. Supported by R01 HL 072748, Luso‐American Foundation fellowship, 155/2007