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Utility of Micro‐Ultrasound and Contrast Agents in the Assessment of a Mouse Model of Renal Ischemic Reperfusion Injury
Author(s) -
Coulthard Tonya,
Andonian Sero,
Lee Benjamin
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.161
Subject(s) - medicine , perfusion , kidney , ultrasound , renal cortex , ischemia , pathology , contrast enhanced ultrasound , acute kidney injury , in vivo , biomarker , nephrology , renal medulla , cortex (anatomy) , renal ischemia , reperfusion injury , radiology , biology , neuroscience , biochemistry , microbiology and biotechnology
High‐resolution micro‐ultrasound enables non‐invasive real‐time quantification of blood velocity measurements, perfusion and endothelial cell biomarkers preclinically. In nephrology research, ischemic reperfusion injury results in extensive damage to both ischemic tissue and systemic tissue in the kidney. This abstract describes the investigation of a mouse model of renal ischemic reperfusion using a dedicated micro‐ultrasound system. Using this novel imaging system, surgical technique was confirmed non‐invasively and perfusion changes within the microvasculature of the contralateral kidney were quantified and the increased expression of P‐selectin was quantified in the kidneys. Results showed a decrease in microvasculature perfusion in the contralateral kidney, the cortex, the medulla, and most dramatically in the cortical‐medullary border zone. An increase in P‐selectin expression as an inflammatory biomarker was observed most dramatically in the cortex and border zone of the ipsilateral kidney. As such, micro‐ultrasound has proven to be a valuable tool for in vivo imaging of mouse models of ischemic reperfusion injury and in quantification of kidney perfusion and inflammatory response.