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Lipoic acid prevents high‐fat diet‐induced hepatic steatosis in Goto Kakizaki rats
Author(s) -
Sena Cristina M,
Louro Teresa,
Nunes Elsa,
Proença Teresa,
Cipriano Augusta,
Seiça Raquel
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.134
Subject(s) - steatosis , medicine , endocrinology , malondialdehyde , oxidative stress , steatohepatitis , inflammation , type 2 diabetes , fibrosis , lipid metabolism , fatty liver , diabetes mellitus , disease
Prevention of fat accumulation in the liver may be an effective therapy for certain diseases. This study was conducted to investigate the effects of the antioxidant α‐lipoic acid (LA) on hepatic steatosis, inflammation and oxidative stress in a model of type 2 diabetes fed with high fat diet (HFD). Goto‐Kakizaki rats were randomly divided into 4 groups. The first group received only standard rat diet (control SD); groups 2 (control HFD), 3 (vehicle group) and 4 (LA group) were given HFD, ad libitum during 3 months. Carbohydrate and lipid metabolism, tumor necrosis factor‐α (TNF‐α), plasma and liver tissue malondialdehyde (MDA), and liver GSH levels were assessed in the different groups. Histopathologically, steatosis, ballooning degeneration, inflammation, and fibrosis were evaluated. Type 2 diabetic animals fed with HFD showed a marked hepatic steatosis fully prevented with LA. No fibrosis or Mallory bodies were found in the hiperlipidemic GK group. Plasma and liver tissue MDA, and hepatic TNF‐α levels were significantly higher in HFD group than in the control group and significantly lower in LA group compared to the vehicle group. No changes in serum transaminases levels were observed in this study. In conclusion, LA strikingly ameliorates steatohepatitis in this animal model of diabetes fed with HFD by inhibiting TNF‐α levels and oxidative stress biomarkers.