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Exercise induces Hsp70 in endothelial cells of the coronary vasculature
Author(s) -
Wolff Steve Michael,
Milne Kevin J,
Noble Earl G
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.125
Subject(s) - hsp70 , heat shock protein , medicine , blot , immunofluorescence , ex vivo , shock (circulatory) , endocrinology , chemistry , cardiology , immunology , in vitro , antibody , biochemistry , gene
Exercise (EX) and heat‐shock (HS) both lead to increased myocardial content of the cardioprotective inducible isoform of the 70 kDa heat shock protein, (Hsp70). Twenty‐four hours after HS, myocardial Hsp70 is co‐localized with blood vessels, indicating a cell‐type specific response to this stress. The objective of the current study was to determine whether a similar localization of myocardial Hsp70 occurs post‐EX. Adult male Sprague Dawley rats were randomized into control (CON), EX (60 min treadmill running, 30 m/min) or HS (42°C, 15 min) groups. Left ventricles (LV) were harvested at 30 min and 24 hrs post‐treatment and analyzed for Hsp70 by both western blotting and confocal immunofluorescence microscopy. Both HS and EX resulted in significant increases in Hsp70 in LV homogenates. Under both conditions, confocal microscopy revealed a minor increase in Hsp70 in the myocytes compared to CON, but as early as 30 min post‐treatment there was a striking increase in endothelial localized Hsp70 that persisted for up to 24 hrs. These data support previous findings with HS and suggest that exercise results in a similar cell‐type specific induction of myocardial Hsp70. Consequently, the cardioprotective nature of exercise may lie in the Hsp70 mediated protection of coronary blood vessels. Supported by CIHR‐CCT‐83029 and NSERC‐8170‐05 RGPIN.