Premium
Analysis of serum proteins differentially expressed during simulated adequate or inadequate secretory phases in the rhesus monkey
Author(s) -
Okulicz William C
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.113
Subject(s) - secretory protein , biology , blood proteins , andrology , gene , biomarker , endometrium , endocrinology , immunology , medicine , genetics
Progesterone has been shown to temporally regulate a number of genes and gene networks in the primate endometrium in order to provide an appropriate milieu for embryo implantation. Our laboratory and other laboratories have documented a number of genes differentially expressed during the expected window of receptivity in nonhuman primates and women. The objective of the current study was to determine whether the expression of serum proteins was associated with fertile or infertile secretory cycles in our rhesus monkey model. Serum samples were obtained 8–10 days following the estradiol peak during simulated adequate versus inadequate (non‐receptive) secretory phases. Samples were subsequently analyzed for differential expression of specific proteins using serum biomarker chips from Schleicher and Schuell Bioscience. In fertile cycles, up‐regulated serum proteins included alpha2 macroglobulin, cathepsin B, E‐selectin and complement C4. Serum proteins over‐expressed during inadequate secretory phases included ErB2, IL2, kallikrein‐14, and G‐CSF. The potential role of these serum proteins in endometrial receptivity remains to be established. The data suggest, however, that differential serum protein composition may be a useful tool as a criteria for fertile or infertile cycles as well as provide important avenues for future studies. Supported in part by NICHD HD31620.