Premium
Investigating Msh2‐Msh3 Function in DNA Mismatch Repair
Author(s) -
Biro Francis Noah,
Hingorani Manju
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.989.8
Subject(s) - msh2 , dna mismatch repair , msh6 , base pair , dna , biology , dna repair , microbiology and biotechnology , genetics , computational biology
DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects errors such as base‐base mismatches and insertion/deletion loops (IDLs) in DNA. MutS proteins (eukaryotic MutS homologues) bind errors in DNA and initiate the repair process. In humans and other eukaryotes, Msh2‐Msh6 complex recognizes base‐base mismatches and small IDLs, and Msh2‐Msh3 complex putatively recognizes both small and large IDLs. Available evidence suggests that Msh2‐Msh3 loop‐binding activity is involved in aberrant triplet‐repeat expansion, which is implicated in such neurodegenerative disorders as Huntington's disease. In order to better understand the function of this protein, we have overexpressed and purified S. cerevisiae Msh2‐Msh3 from E. coli cells . We are currently measuring the kinetic and thermodynamic parameters that govern Msh2‐Msh3 DNA binding and ATPase activity and define its mechanism of action in MMR.