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SYNTHESIS OF ESTROGENS AND ANDROGENS IN THE GONADS OF THE FETAL SHEEP
Author(s) -
Gomez Yolanda,
Infante Veronica Chavez,
Ramirez Esther Bautista,
Pardo Marta Romano
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.986.1
Subject(s) - medicine , endocrinology , fetus , secretion , radioimmunoassay , testosterone (patch) , estrogen , biology , gonad , androgen , aromatase , estrone , chemistry , hormone , pregnancy , cancer , breast cancer , genetics
In this work we investigated the secretion of 17B‐estradiol (E2) and testosterone (T) in the gonads of ovine fetuses of 3.6 and 12 inches in crown‐rump (C‐R) length. The fetus were obtained immediately after the slaughtering of animals and transferred to phosphate‐buffer in cold. The gonads were carefully dissected and cells were obtained by dissociation trypsin treatment, the cells isolated were incubated in Dulbecco's medium plus 0.1 BSA during one hour to 370C. The secretion of E2 and T were measured by radioimmunoassay in the incubation medium of the cells. The 17B‐ estradiol was the predominant estrogen produced by ovaries during the developing sheep gonad. The fetuses of 12 in showed the highest secretion of E2 58pg/mg protein whereas, the minimum secretion level of E2 were 10 pg/mg protein in the fetuses of 8 in. The amount of testosterone in these ovaries was not detectable in the previous fetuses until the 8 in fetuses an average of 15pg/mg protein and the secretion was maintain during all fetuses development. Testosterone was the principal androgens synthesized by the testes. The maximum level of T secretion 600pg/mg protein was found in the feto 6.4 in. By the other hand, the E2 secretion was low during the process. These results show that during the development of the ovine fetal gonads synthesizes estrogens and androgens, which strongly suggests the activity of P450‐aromatase and 17B‐HSD. CONACYT 41423 Q