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Glomerular cell density and number in OVE26 diabetic mice
Author(s) -
Carlson Edward C,
Teiken Jennifer,
Kees Eric,
Audette Janice,
Laturnus Donna,
Zheng Shirong,
Epstein Paul N
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.982.2
Subject(s) - medicine , endocrinology , mesangial cell , podocyte , diabetes mellitus , genetically modified mouse , cell , muscle hypertrophy , chemistry , biology , transgene , kidney , proteinuria , biochemistry , gene
We recently showed that in OVE26 transgenic diabetic mice, glomerular volume was increased while podocyte density (pN V ) and number (pN) were reduced, particularly in older animals. In the current study, similar LM and TEM morphometric techniques were applied to OVE26 and age‐matched FVB control mice (60, 150, or 450 days) in an effort to determine whether endothelial and mesangial cell density (eN V , mN V ) and number (eN, mN) also may be altered and how the data may relate to total glomerular cell density and number. At 60 days, mN V , eN V , mN, and eN were not statistically distinguishable in OVE26 and controls. Likewise, at 150 days, eN V and mN V were not significantly altered in the diabetics. However, at 150 days, both mN and eN were significantly increased (~143% and ~62% respectively), and in 450 day‐old diabetic animals, mN V , mN and eN were all significantly increased (~78%, ~179%, and ~71% respectively) relative to age‐matched FVB mice. These data indicate that in older OVE26 mice, mesangial and endothelial cells numbers increase leading to significant increases in total glomerular cell number, and that glomerular hypertrophy is accompanied by both mesangial and endothelial cell proliferation.