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Echocardiography under isoflurane anesthesia affects heart rate and function in commonly utilized mouse strains
Author(s) -
Rojas Mauricio,
Meredith Dane,
Kylander Jackie,
Barrick Delia,
Corn Drew,
Lockyer Pamela,
Patterson Cam,
Willis Monte S
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.970.44
Subject(s) - isoflurane , anesthesia , medicine , heart rate , cardiac function curve , cardiology , blood pressure , heart failure
While many studies have described the advantages of performing echocardiography in mice using isoflurane anesthesia over other anesthetics, the direct comparison of isoflurane and conscious echocardiography in common mouse strains has not previously been determined. In the present study, we compared cardiac function in two commonly used mouse strains (C57BL/6 and 129S) at 8 weeks of age using high resolution two‐dimensional echocardiography (30MHz). C57BL/6J (N=12) and 129S (N=12) were echoed both under anesthesia (1.5% isoflurane in 100% oxygen) and consciously by four different operators within 1 week. We identified significant decreases in heart rate in anesthetized mice (both strains) compared to conscious mice (620+/−66 vs. 466+/−54). Since heart rate affects cardiac function, it was not surprising that a corresponding decrease in fractional shortening % was also identified in both strains (54%+/−11 vs. 35%+/−10) under the same conditions. Both anterior and posterior wall measurements as well as interventricular distances did not significantly differ between echo methods or individual operators. These findings demonstrate the utility of echocardiographic assessment of cardiac function in both conscious and isoflurane anesthetized mice in multiple commonly used mouse strains, highlighting the specific parameters affected by isoflurane.