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Collagen metabolism and renal damage in 2k1c rats
Author(s) -
Skogstrand Trude,
Hultström Michael,
Iversen Bjarne M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.968.5
Subject(s) - western blot , renal cortex , kidney cortex , endocrinology , medicine , kidney , superoxide dismutase , cortex (anatomy) , gene expression , metabolism , fibrosis , chemistry , real time polymerase chain reaction , gene , biology , biochemistry , oxidative stress , neuroscience
We have found a clear difference in gene expression for the enzymes of collagen metabolism between the inner and outer renal cortex in old spontaneously hyptertensive rats with renal damage. The progress of fibrosis in 2k1c rats is less well studied. In the present study we have investigated 2k1c rats six weeks after clipping using western blot and real time PCR to compare the gene and protein expressions in the inner and outer cortex. Using real‐time PCR we studied collagen type‐1‐A1, procollagen‐n‐ and ‐c‐peptidase, MMP‐2, MMP‐9, TIMP‐1 and ‐2. This was complemented with protein quantification using western blot for MMP‐9 and TIMP‐2 as well as the superoxide dismutases EC‐SOD and MN‐SOD. No differences were found between the inner and outer cortex of the kidney for any of the genes and no difference was found between the expressions in 2k1c and controls. This while the systolic pressure after six weeks was 126±2 mmHg in controls and 150±4 mmHg in the 2k1c rats. Our conclusion is that: While 6 weeks is pleny of time for hypertension to develop, it is not enough for the collagen metabolism to be significantly altered at the gene expression level. The study was founded by Helsevest, Norway.