Premium
A new and simple method for detecting non‐invasive vascular endothelial function in mice
Author(s) -
Furuta Tomoaki,
Matsushita Shonosuke,
Sugimori Haruhiko,
Sato Fujio,
Sakakibara Yuzuru
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.967.10
Subject(s) - vasodilation , reactive hyperemia , nitric oxide , chemistry , hindlimb , laser doppler velocimetry , blood flow , medicine
Purpose: The importance of non‐invasive measuring of vascular endothelial function is widely recognized. However, it has not been established in mice as far as we know. From this background, we tried to establish a new method to measure a vascular endothelial function through nitric oxide (NO) mediated vasodilation. Method: Mouse hindlimb (n = 6) was compressed for a short time (30 seconds) and released immediately. The arterial blood flow was measured continuously using laser Doppler flow meter at arcus plantaris. Result: Reactive hyperemia (127+/−10% from baseline) at reperfusion was reproducibly‐observed. This reaction was mostly inhibited by L‐NAME (5mg/kg i.p.) (107+/−5%, p<0.005 vs. without L‐NAME). And it was completely‐recovered by supplying exogenous NO (122+/−16%, p<0.05 vs. after L‐NAME). Therefore, this method was considered to reflect NO‐mediated endothelial function. On the other hand, this was not inhibited by non‐selective cyclooxygenase inhibitor indomethacin (IDM; 20 mg/kg i.p.) (baseline: 120+/−11 vs. after IDM: 128+/−10%, p:NS). Conclusion: From these results, we confirm that this method is a new and simple technique measuring NO‐mediated endothelial function non‐invasively in mice.