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AMP Kinase (AMPK) activator 5‐aminoimidazole‐4‐carboxamide‐1‐β‐D‐ribofuranoside (AICAR) alters vascular smooth muscle vasomotor responses in thoracic aorta rings from spontaneously hypertensive (SHR) and Wistar‐Kyoto (WKY) rats
Author(s) -
Ford Rebecca J,
Rush James W.E.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.965.6
Subject(s) - endocrinology , medicine , ampk , phenylephrine , vasomotor , chemistry , vasoconstriction , vascular smooth muscle , spontaneously hypertensive rat , activator (genetics) , electrical impedance myography , vasodilation , protein kinase a , kinase , smooth muscle , receptor , blood pressure , biochemistry
The metabolic regulatory role of AMPK is well established but potential mechanisms of AMPK in vascular control are not well defined. Aortic rings were isolated from 16–20wk old male SHR and WKY rats and assessed using myography to determine potential vasomotor effects of AICAR. There was a significant drop in resting tension after endothelium‐denuded rings were incubated with AICAR (A; 2mM, 30 min) in SHR (control (C) = −0.14±0.06g, A = −0.97±0.09g, p<0.01) but not in WKY vessels (C = −0.26±0.06g, A = −0.24±0.08g, p=NS, strain*drug p<0.01). Both SHR and WKY denuded vessels relaxed upon exposure to AICAR following phenylephrine pre‐constriction (PE 10 −7 M; SHR: C = 2.1±2.2%, A = 41.4±11.9%, p<0.01; WKY: C = 3.0±2.2%, A = 23.7±9.7%, p<0.01). In denuded vessels pre‐exposed to AICAR, potassium chloride dose‐response curves were right‐shifted in both SHR and WKY (SHR C vs A p<0.01, WKY C vs A p<0.01) and developed tension to increasing PE concentrations was blunted in both strains at all concentrations (max tension SHR: C = 2.07±0.19g, A = 1.14±0.21g, p<0.01; WKY: C = 2.57±0.19g, A = 1.98±0.21g, p<0.01, strain*drug p<0.01). These results suggest that AMPK can influence vasomotor function in SHR and WKY aortic rings. Funded by the Heart and Stroke Foundation of Ontario