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Exercise training decreases the size and alters the composition of the neointima in a porcine model of coronary restenosis
Author(s) -
Fleenor Bradley Stephen,
Ivey Jan,
Casati Jennifer,
Bowles Douglas K.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.965.16
Subject(s) - neointima , restenosis , medicine , angioplasty , artery , lesion , cardiology , circumflex , coronary arteries , right coronary artery , surgery , stent , myocardial infarction , coronary angiography
Exercise training reduces adverse events following coronary angioplasty in humans. Therefore, our aim was to determine if exercise reduces the size or alters the composition of the neointima (NI) in a porcine balloon coronary injury (BCI) model. Miniature Yucatan swine were randomly assigned to cage confinement (SED, n=7) or exercise (EX, n=8) training on a treadmill. After 4 months the animals underwent BCI of the left anterior descending (LAD) and left circumflex (LCX) arteries. The animals recovered for 2 days and resumed the previous protocol of SED or EX. Additionally on day 12 following the BCI, each animal received an intravenous injection of BrdU to label proliferating cells. At 28 days post BCI the animals were euthanized and the LAD and LCX were excised. Injured and uninjured sections from each artery underwent standard histological procedures and were stained with VVG, pico Sirius red and antibodies for BrdU and fibronectin. Our results demonstrate that exercise decreased lesion size and NI proliferation (−48%) in the LAD (p<.05) but not the LCX. Exercise significantly increased NI collagen (5.9%) and decreased NI fibronectin (−7.9%) in both arteries (p<.05). However, in the uninjured media collagen decreased in the EX (−5.2%, p<.05) with no effect on fibronectin. In conclusion, exercise may decrease post angioplasty events by reducing lesion size and altering NI composition. Supported by: NIH HL 52490