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Profile of E‐cadherin mobility in the endothelial junction
Author(s) -
Quadri Sadiqa K,
Sun Li,
Bhattacharya Jahar
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.964.33
Subject(s) - cadherin , adherens junction , ve cadherin , fluorescence recovery after photobleaching , confocal , perimeter , chemistry , biophysics , tight junction , photobleaching , cell junction , microbiology and biotechnology , fluorescence , biology , cell , physics , biochemistry , optics , geometry , mathematics
The adherens junction protein, E‐cadherin determines lung endothelial (EC) barrier properties (2003. 278:13342‐9). To determine EC cadherin dynamics, we over expressed full length of GFP‐E‐cadherin in confluent monolayers of rat lung microvascular EC (RLMEC). Viewed by confocal microscopy, E‐cadherin fluorescence (Io) was patchy, being high (>100 grey levels) in some regions, but low (<100 grey levels) in 75±5% of the cell perimeter (mean±SE; n=10 cells). To determine E‐cadherin mobility, at different sites along the EC perimeter we determined the fluorescence recovery after photobleaching (FRAP) to 50% of Io. To rule out errors due to non‐specific bleaching, we corrected FRAP against fluorescence of reference landmarks. At regions of low E‐cadherin expression, FRAP was 40±6% of Io in 2.5 min. At regions of high E‐cadherin expression, FRAP was slower, being 27±4% of Io (P<0.01). We conclude that in a substantial part of the EC perimeter, E‐cadherin expression is relatively low and that in these regions E‐cadherin mobility is higher than in regions of high E‐cadherin expression. We speculate that E‐cadherin mobility decreases as the junctions stabilize and endothelial barrier properties increase (Support: HL36024).