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PAF and H2O2 act synergistically to increase endothelial cell oxidant stress
Author(s) -
Wang Zhanfei,
Bian Zhouyan,
Rui Tao,
Kvietys Peter R.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.964.30
Subject(s) - catalase , nadph oxidase , chemistry , endothelial stem cell , oxidative stress , anti oxidant , antagonist , pharmacology , receptor , biochemistry , microbiology and biotechnology , biology , antioxidant , in vitro
We have previously shown that alveolar macrophages from septic mice can 1) induce an oxidant stress in pulmonary endothelial cells (PEC) and 2) promote neutrophil (PMN) transendothelial migration. Both of these responses can be blocked by catalase or a PAF receptor antagonist. In the present study, we assessed whether PAF and/or H2O2 can induce an oxidant stress in endothelial cells. PEC were isolated from murine lungs and exposed to different concentrations of H2O2 (25 – 200 μM) and PAF (10‐10 – 10‐7 M) for 1 hr. Subsequently, PEC oxidant stress was assessed using DHR 123 oxidation as an index. H2O2 increased PEC oxidant stress only when used at the highest concentration (200 μM), while PAF did not increase PEC oxidant stress at any of the concentrations tested. When PAF and H2O2 were added simultaneously to PEC there was a potentiation of PEC oxidant stress. Our findings indicate that PAF and H2O2 act synergistically to promote endothelial cell oxidant stress, most likely through the activation of NADPH oxidase.