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Dynamic motion of paxillin on actin filaments in living endothelial responses to shear stress
Author(s) -
Hu YingLi,
Chien Shu
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.964.28
Subject(s) - paxillin , microbiology and biotechnology , focal adhesion , actin , cytoskeleton , chemistry , signal transducing adaptor protein , live cell imaging , confocal , actin cytoskeleton , biology , signal transduction , cell , biochemistry , geometry , mathematics
Paxillin, an adaptor protein that is associated with the cytoplasmic domains of integrins and localized at focal adhesions (FAs), interacts with cytoskeletal and signaling proteins to regulate the assembly, disassembly, and signaling of FAs. In living endothelial cells (ECs), fluorescence imaging of the intracellular dynamics of paxillin and actin filaments indicated that actin filaments are important in paxillin assembly at FAs. Using a laser scanning confocal microscopic imaging system, three‐dimensional (3‐D) images were obtained from a z‐series collection of dual‐color optical sections of fixed cells. The results showed the co‐localization of paxillin on actin filaments as fibrous structures, as well as clusters. In living ECs under flow condition, concurrent monitoring of the intracellular dynamics of DsRed2‐paxillin and GFP‐actin by time‐lapse video recording and dual‐color fluorescence imaging showed that the paxillin fibrous structures in ECs moved in close association with and along the actin filaments. Our findings suggest that the actin network not only plays an important role in the assembly/disassembly of paxillin at FAs, but also serves as a track for the intracellular transport of paxillin, which plays an important role in cellular signaling cascades. This work was supported by National Heart, Lung, and Blood Institute Research Grants HL‐064382, HL‐080518, and HL‐085159 (S.C.).