Premium
Effect of 4 days of intermittent hypoxia on oxidative stress in healthy men
Author(s) -
Pialoux Vincent,
Foster Glen E,
Brugniaux Julien V,
Duggan Cailean T,
Hanly Patrick J,
Poulin Marc J
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.960.3
Subject(s) - intermittent hypoxia , oxidative stress , medicine , endocrinology , hypoxia (environmental) , lipid peroxidation , obstructive sleep apnea , reactive oxygen species , chemistry , oxygen , biochemistry , organic chemistry
Periodic occlusion of the upper airway in patients with obstructive sleep apnea (OSA) leads to intermittent hypoxia (IH). IH is characterized by repeated episodes of hypoxia/reoxygenation, which is known to increase the production of reactive oxygen species (ROS). Causal relationships between OSA, IH and oxidative stress have not been established because of confounding effects of medical co‐morbidities and obesity which are frequently associated with OSA. The aim of this study was to determine if IH changes oxidative stress in healthy volunteers. Nine men (29.3 ± 1.7 years; BMI, 25.0 ± 0.4 kg.m −2 ; mean±SD) were studied. Following baseline measurements breathing room air (day zero (D0)), all subjects were exposed to 4 days (D1 to D4) of IH while awake (2 min at P et O 2 = 45 Torr and 2 min at P et O 2 = 88 Torr) 6 hours.d −1 . Lipid peroxidation (malondialdehides, MDA), DNA oxidation (8‐Hydroxy‐2′‐deoxyguanosine, 8‐OHdG) and oxidation protein products (AOPP) were assessed in plasma. MDA increased significantly between baseline (D0) and D1 (+39%: 3.48 ± 1.17 vs 4.85 ± 0.90 μmol.l −1 ) and then tended to return to baseline l evels. 8‐OHdG increased significantly between D0 and D1 (+51%: 53.84 ± 54.63 vs 83.30 ± 45.49 μmol.l −1 ) and remained elevated until D4 (+67%: 53.84 ± 54.63 vs 89.71 ± 36.06 μmol.l −1 ). These findings demonstrate that IH increases oxidative stress and suggest that IH may contribute to ROS overproduction in OSA. Supported by the Alberta Heritage Foundation for Medical Research and the Heart & Stroke Foundation of Canada.