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Metabolic effects of electrical stimulation in C2C12 myocytes
Author(s) -
Philp Andrew,
Donnelly Kenny,
Baar Keith
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.959.22
Subject(s) - mitochondrial biogenesis , ampk , stimulation , coactivator , phosphorylation , peroxisome proliferator activated receptor , mitochondrion , amp activated protein kinase , endocrinology , medicine , chemistry , peroxisome , microbiology and biotechnology , beta oxidation , transcription factor , biogenesis , biology , receptor , metabolism , protein kinase a , biochemistry , gene
Members of the Peroxisome proliferator‐activated receptor (PPAR)‐γ coactivator (PGC) family are believed to control cellular metabolism and mitochondrial biogenesis. PGC‐1α is the most characterized of the family members with gain of function studies suggesting a role in slow fiber transition, mitochondrial biogenesis and glucose and fatty acid metabolism. Less is known about the role, if any, of the other two family members, PGC‐1β and PRC. The aim of this study was to investigate the activation of the PGC‐1 family members following electrical stimulation. Three hours of 1Hz stimulation resulted in an 81% increase in CaMKII (Thr 286) phosphorylation (P=0.003), a 43.5% increase in AMPK (Thr 172) phosphorylation (P=0.04) and a 23.1% increase in PKA (Thr 197) phosphorylation (P=0.12). None of the PGC family members were transcriptionally activated immediately following stimulation. PGC‐1α transcription peaked (4.37‐fold increase) at 6 hours whereas PGC‐1β and PRC were not significantly increased at any time point. These data indicate that low frequency electrical stimulation induces the activation of CaMKII, AMPK, and PKA and induces the transcription of PGC‐1α. This model is well suited for rapid identification of factors that are essential in the activation of PGC‐1α and mitochondrial biogenesis.