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Exercise‐induced modulation of renal vs. systemic endothelium
Author(s) -
Moningka Natasha,
Ahlgren Joslyn,
Hayward Linda,
Baylis Chris
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.957.13
Subject(s) - enos , medicine , endocrinology , ex vivo , kidney , chemistry , oxidative stress , endothelium , superoxide dismutase , nitric oxide , nitric oxide synthase , biochemistry , in vitro
Endothelial nitric oxide (NO) synthase (eNOS) and extracellular (EC) superoxide dismutase (SOD) are increased in exercise (EX) due to increased shear stress, but in kidney, blood flow is reduced with EX. We investigated the impact of EX on renal and aortic eNOS and SOD. Rats were subjected to 6wks voluntary running wheel EX (EX, n=7; sedentary (SED), n=3). At wk 6, average distance run=4.3±1.6 km/day. Decreases in HR and MAP (by telemetry) were seen in EX rats. Western blots of renal cortex (RC) revealed falls in eNOS and EC SOD protein abundance, while aortic eNOS was unchanged and EC SOD increased with EX (Table). In contrast, there were no significant changes in Mn SOD with EX while Cu/Zn SOD increased with EX in RC. Protein abundance measurements expressed in units of IOD/Ponceau red/Int Std; *p<0.05 vs. SED. The marked reduction in eNOS abundance and tendency for EC SOD to fall with EX in the kidney is predictable based on the reduction in renal blood flow. The increased RC Cu/Zn SOD may help to buffer exercise‐induced oxidative stress but the decreased eNOS will likely compromise endothelium‐dependent renal vasodilation. The long term impact of EX on kidney function and structure in health and in renal disease requires further study.