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Redox Regulation of Circulating NO Bioavailability & Post‐Exercise Hemodynamics in Hypertension
Author(s) -
New Karl James,
Bailey Damian,
McEneny Jane,
Templeton Catherine,
Ellis Gethin,
James Philip,
Davies Bruce
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.957.12
Subject(s) - ascorbic acid , medicine , hemodynamics , oxidative stress , chemistry , bioavailability , endocrinology , vitamin c , vascular resistance , cardiology , pharmacology , food science
9 pre‐hypertensive males, MAP = 106 ± 5 mmHg were studied for 2‐hours following 30‐minutes of cycle exercise at 70% maximal oxygen consumption. Echocardiography determined cardiac output (CO) and systemic vascular resistance (SVR) by MABP/CO. Venous blood was sampled from an antecubital vein pre‐, immediately post‐, 1‐hour post‐ (P1) and 2‐hours post‐ (P2) exercise and corrected for plasma volume shifts. Plasma nitrate (NO − 3 ) and nitrite (NO − 2 ) was determined fluorometrically, S‐Nitrosothiol (RSNO) concentrations were assayed by the Saville reaction. Lipid hydroperoxides (LOOH) were determined spectrophotometrically whilst blood was also assayed for selective antioxidants. Upper (UL) and lower limb (LL) arterial pulse wave velocity (APWV) was determined pre and post‐exercise via oscillometry. Ascorbic acid, α‐tocopherol, RSNO and LL APWV were unchanged. UL APWV was 18% lower than baseline at post recovering by P2 being 9% elevated above baseline. Elevated oxidative stress in the recovery period coincides with a blunting of circulating NO, UL arterial compliance and a return of SVR towards baseline.