Premium
Nicotinic acid (NA) alters lipid metabolism in a pair‐fed mouse model
Author(s) -
White Amanda T,
Menke John,
Krsmanovic Mihajlo,
Wilsie Larissa,
Taggart Andrew K P,
Strack Alison,
Metzger Joseph
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.948.5
Subject(s) - medicine , endocrinology , chemistry , meal , respiratory quotient , cholesterol
Pharmacological doses (2–3 grams/day) of NA favorably alter the serum lipid profile by increasing HDL while decreasing VLDL and LDL cholesterol and triglycerides (TG). In rodents, the poor pharmacokinetic properties of NA (plasma half‐life on the order of minutes) necessitates admixture in chow to deliver sustained plasma exposures. In C57BL/6 mice treated with NA diet at 1 or 3% (w/w) in chow, TG levels are lowered after two days of treatment. However, food intake (FI) is reduced during this period, which could artificially decrease TG levels in NA‐treated mice by inducing a fasted state. To separate the mechanism‐based effect of NA from aversive effects of the diet we utilized a CLAM pair‐feeding (PF) system with indirect calorimetry to yoke the FI of the PF group to the NA‐treated group in 30′ intervals. Exposure to 3% NA diet altered meal pattern and decreased FI by 57, 35, 11 and 0 % on days 1–4. Body weight decreased by 17.1 % in NA‐treated mice while the PF group lost only 8.9 % (day 4, v. ad lib controls). Serum TG levels on day 4 in ad lib and PF animals did not differ (119 and 118 mg/dL, respectively) whereas NA treatment decreased serum TG by 29% (85 mg/dL). These data show that the NA effects on TG are independent of the suppression of FI. Interestingly, mice treated with NA had decreased respiratory quotient even when their FI matched that of vehicle animals, suggesting an altered use of glucose and/or an increase in beta‐oxidation.