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Estimating Circadian Sleep/Wake Cycle From Rest/Activity Behaviors In Inbred Strains Of Rats
Author(s) -
Feroah Thom R,
Ver H J,
McQuestion G,
Hutchins W,
Forester H V,
Ferri R,
Manconi M,
Merritt A,
Dwinell M,
MorenoQuinn C,
Cowley A,
Jacob H
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.946.2
Subject(s) - circadian rhythm , sleep (system call) , free running sleep , sleep onset , endocrinology , rhythm , actigraphy , medicine , inbred strain , congenic , biology , psychology , insomnia , circadian clock , psychiatry , genetics , gene , computer science , light effects on circadian rhythm , operating system
To establish methods to screen inbred rat strains, we studied EEG/EMG and movement in Brown Norway (BN MCWi ), Fawn Hooded (FH MCWi ), and Dahl Salt Sensitive (SS MCWi ) inbred rat strains in environmental chambers during 3 successive 12L:12D circadian light:dark cycles. The 3 rd light:dark cycle was analyzed for the circadian relationship of the onset, offset and duration of sleep state and movement. Although circadian amplitude and phase onset of sleep was different* between strains, onset, offset and duration of sleep and movement were significantly* correlated within strains. Analyzing probability of sleep onset and sleep offset from duration of inactivity we established a 95% probability* of sleep after 35 sec and 0.75 sec, respectively, that was NOT different* within and between strains. We developed an algorithm to determine sleep/wake periods from movement in previously unstudied FH MCWi and BN MCWi rats and found we correctly* estimated sleep onset (90%) and sleep offset (96%) in all observations. We have revised the algorithm to estimate 4 periods of sleep/wake (Active Wake, Quiet Wake, Sleep and Sustained Sleep) to further characterize sleep/wake. In conclusion we have established methods to screen sleep/wake patterns in consomic, congenic and ENU mutated inbred rat strains and to investigate physiological dysfunction and sleep in these models. This study was funded by NHLBI (U01 HL66579) (*P < 0.05)