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Effects of Caffeine on Exercise Fatigue in Sleep Deprived Mice
Author(s) -
Zielinski Mark R.,
Carmichael Martin D.,
Murphy E. Angela,
Youngstedt Shawn D.,
Davis J. Mark
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.946.1
Subject(s) - caffeine , sleep deprivation , sleep (system call) , placebo , endocrinology , medicine , privation , treadmill , sleep loss , circadian rhythm , psychology , computer science , operating system , alternative medicine , pathology
In humans, exercise run‐time to fatigue is reduced following sleep deprivation. Caffeine can be effective in reducing fatigue in many conditions, but there is no evidence regarding the effect of caffeine on run‐time to fatigue following sleep deprivation. We have developed an animal model of sleep deprivation and fatiguing exercise in order to better address potential related CNS mechanisms. Twenty‐four male CD‐1 mice were randomly assigned to 4 conditions in a 2 SLEEP (normal sleep or sleep deprivation) X 2 SUPPLEMENT (caffeine or placebo) experimental design. Sleep deprivation groups were placed on a slow rotating disc for 24 h to prevent sleep. Normal sleep groups were concurrently placed on the same apparatus, but with the disc locked so that sleep was allowed. Mice received an i.p. injection of either caffeine (6 mg/kg) or placebo (saline) 30 min prior to exercise. Immediately after sleep deprivation or normal sleep, mice ran on a treadmill (24 m/min) until fatigue. Then, the mice were sacrificed and plasma was collected for glucose analysis. Sleep deprivation significantly reduced run‐time to fatigue (p < 0.05) and caffeine attenuated this effect (p < 0.05). No significant treatment differences in plasma glucose were found. Further research is planned to address potential CNS mechanisms in this animal model.