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Differential Phenotype of Placodal vs Neural Crest Derived Afferent C‐fibers in Airways and Esophagus.
Author(s) -
Undem Bradley J.,
Kwong Kevin,
Nassenstein Christina,
Kollarik Marian
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.945.2
Subject(s) - nodose ganglion , neural crest , dorsal root ganglion , biology , ganglion , anatomy , trpv1 , nociceptor , neuroscience , receptor , microbiology and biotechnology , pathology , nociception , vagus nerve , embryo , medicine , spinal cord , transient receptor potential channel , stimulation , biochemistry
C‐fibers in the guinea pig respiratory tract comprise two distinct phenotypes, depending on whether the cell body is situated in the nodose (placodal origin) ganglion or jugular (neural crest origin) ganglion (Undem et al. J.Physiol, 2004). Both types of C‐fibers are responsive to capsaicin but only the nodose C‐fibers are responsive to purinergic P2X receptor, 5‐HT‐3 receptor, and adenosine A1 receptor agonists. The vast majority of jugular, but not nodose ganglia express neurokinins. We speculate that the C‐fiber phenotype may be determined more by their embryonic source than from molecular cues arising from the tissue they innervate. To further evaluate this, we compared lung and esophageal C‐fiber neurons arising from dorsal root ganglion (DRG), nodose, and jugular ganglia, based on the knowledge that the DRG neurons innervating these tissues are derived from the same embryonic source as the jugular neurons. Neurons labeled from the lungs and studied with single cell RT‐PCR, histology, and whole cell patch clamp recordings, revealed that DRG and Jugular C‐fiber phenotype are equivalent, but quite distinct from the nodose C‐fibers. A similar distinction was observed in esophageal‐specific afferent neurons. The results indicate that the C‐fiber phenotypes innervating the lungs and esophagus are determined more by their embryonic environment than by the environment of the tissue they ultimately innervate.