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Dietary omega‐3 fatty acids prevent nitric oxide synthase loss in diabetic kidney disease
Author(s) -
Garman Joe,
Xu Qin,
Maric Christine
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.944.11
Subject(s) - enos , medicine , endocrinology , diabetes mellitus , chemistry , kidney , blood pressure , glomerulosclerosis , nitric oxide , nitric oxide synthase , proteinuria
Background: We previously showed that a diet rich in omega‐3 fatty acids attenuates albuminuria, glomerulosclerosis and tubulointerstitial fibrosis associated with diabetes. The aim of the present study was to further examine the mechanisms by which omega‐3 fatty acids exert their renoprotective effect. Methods: The study was performed in 10 week‐old male Sprague‐Dawley non‐diabetic (ND) and streptozotocin‐induced diabetic rats fed normal rat chow (D), or rat chow supplemented with canola oil which is rich in omega‐3 fatty acids (DC) for 30 weeks. Blood pressure was examined by tail cuff sphygmomanometry. Results: D was associated with an increase in systolic blood pressure (BP; ND 153±9; D 198±7; mmHg, p<0.05), decrease in eNOS protein expression in kidney cortex (KC) (eNOS ND 1.29±0.28; D 0.41±0.05, P<0.01) and eNOS and nNOS in red blood cells (RBC) (eNOS ND 1.16±0.12; D 0.27±0.06, P<0.001, nNOS; ND 0.92±0.05; D 0.54±0.10, P<0.05). Diet supplemented with canola oil prevented these changes (BP 162±9; eNOS RBC 1.10±0.07; nNOS RBC 1.48±0.10; eNOS KC 1.50±0.10). No changes were seen in iNOS. Conclusion: Our results show that diet rich in omega‐3 fatty acids prevents the decrease in eNOS and nNOS in the diabetic kidney. These data suggest that a diet rich in omega‐3 fatty acids is beneficial in attenuating hypertension and protects the kidneys by preserving eNOS and nNOS protein expression.