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Oxidative stress does not mediate hypertension and renal injury in ovariectomized DS rats
Author(s) -
LopezRuiz Arnaldo,
Zhang Huimin,
Reckelhoff Jane
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.940.5
Subject(s) - ovariectomized rat , medicine , endocrinology , oxidative stress , estrogen , chemistry , kidney , blood pressure , excretion
Blood pressure (BP) increases in postmenopausal women. BP is higher in ovariectomized (OVX) Dahl salt sensitive rats (DS) compared to intact females (INT). Estrogen is an antioxidant. We hypothesized that OVX leads to increased oxidative stress (ROS), increased BP and renal injury. INT and OVX, 13 weeks of age (n=4–6/grp), were given low salt (LS) diet 0.3%, and supplemented with either tempol (T) (1mmol/L, p.o.) or water for 2 weeks. BP was measured via femoral catheter. ROS was measured by lucigenin in kidney. Urinary albumin (UAlb) and nitrate/nitrite excretion (UNOx) were measured at the end. OVX on LS had higher MAP than INT, and T had no effect in either group. ROS was higher in OVX than INT, and was reduced with T in OVX. UAlb was higher in OVX, and was not affected by T in either group. UNOx was higher in INT than OVX, and was increased with T only in OVX. Despite the fact that ROS are higher and UNOx increases with T in OVX, ROS play no role in mediating HT or renal injury in OVX DS rats on LS. These data suggest that antioxidants may not reduce BP or renal injury in postmenopausal women. (Supported by NIH HL66072)