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Female mRen2.Lewis Exhibit Higher Intrarenal Renin But Lower Renal and Circulating Angiotensin II Than Male Littermates
Author(s) -
Pendergrass Karl,
Chappell Mark C
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.940.4
Subject(s) - endocrinology , medicine , renin–angiotensin system , plasma renin activity , chemistry , kidney , angiotensin ii , neprilysin , blood pressure , enzyme , biochemistry
The mRen2.Lewis rat (mRen), a congenic hypertensive model, exhibits marked gender differences in blood pressure and tissue injury. Male mRen have 2‐fold higher plasma and renal levels of Angiotensin II (Ang II) compared to female mRen or Lewis controls. Plasma Ang I was also higher in male mRen, but renal Ang I content was significantly reduced. Cortical ACE activity was similar for male and females, however, renal neprilysin protein was 10 fold higher in female mRen possibly contributing to the metabolism of intrarenal Ang II. The present studies further characterized gender differences in the mRen focusing on the expression of renin. Rat renal renin levels (pH 6.5) were 55% [M: 3.6±1 vs. F: 8.0±2 μg AngI/mg/hr; p≤0.01] and 80% [M: 0.5±0.1 vs. F: 2.9±0.3 μg AngI/mg/hr; p≤0.01] lower in males versus females for Lewis and mRen rats, respectively. Female rats also displayed higher mouse renin levels (pH 8.5). Correlation analysis of all groups revealed a negative association between renal renin and plasma Ang II [r 2 =0.4, p≤0.05], but not renal Ang II [p≥0.05]. Negative feedback of renal renin is evident in the mRen strain, although the mechanism for gender differences in renin regulation is not known. Moreover, the greater Ang II content in the male mRen kidneys may arise from a nonrenal source. Supported by NIH grants HL56973 & HL51952.