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Endothelial dysfunction in pulmonary arteries from diabetic rats
Author(s) -
LopezLopez JG,
Moral J,
Frazziano G,
GómezVillalobos MJ,
FloresHernández JV,
Flores G,
GutiérrezGodinez J,
PerezVizcaino F
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.915.1
Subject(s) - apocynin , endothelial dysfunction , nadph oxidase , medicine , endocrinology , diabetes mellitus , superoxide , vasodilation , endothelium , sodium nitroprusside , nitric oxide , chemistry , oxidative stress , biochemistry , enzyme
Diabetes mellitus has been recently associated to a higher prevalence of pulmonary hypertension. Diabetes leads to multiple cardiovascular alterations, including endothelial dysfunction in multiple vascular beds. However, the effects of diabetes on the pulmonary circulation are unknown. We analyzed the endothelial function in isolated pulmonary arteries from rats with streptozocin‐induced diabetes. Diabetes led to endothelial dysfunction as denoted by a marked decrease in the endothelium‐dependent vasodilatation induced by acetylcholine in pulmonary arteries from streptozocin‐treated compared to control rats. Acetylcholine‐induced relaxations were abolished by the NO synthase inhibitor L‐NAME. The contractile responses to L‐NAME were also reduced in arteries from diabetic rats. However, the endothelium‐independent vasodilatation to sodium nitroprusside was similar in both groups. The endothelial dysfunction was reverted by incubating the arteries with superoxide dismutase or the membrane NADPH oxidase inhibitor apocynin. Endothelial dysfunction was associated with an increase the production of superoxide as measured by dihydrotheidium and an increased expression of the NADPH oxidase subunit p47phox. In conclusion, we show for the first time that diabetes induced endothelial dysfunction in pulmonary arteries due to enhanced NADPH oxidase‐derived superoxide production