Premium
Differential regulation of oxidative stress by NO in vascular and nonvascular tissues of endotoxemic rats
Author(s) -
SahanFirat Seyhan,
Canacankatan Necmiye,
Korkmaz Belma,
Yildirim Hatice,
Tamer Lulufer,
Buharalioglu C Kemal,
Sari A Nihal,
Tunctan Bahar
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.912.24
Subject(s) - spleen , kidney , oxidative stress , medicine , endocrinology , nitrite , aorta , superior mesenteric artery , superoxide dismutase , chemistry , organic chemistry , nitrate
Endotoxemic shock is a systemic inflammatory response that is associated with increased NO production by iNOS which contributes to the fall in blood pressure (BP), vascular hyporeactivity and multiple organ failure. NO has also been reported to have both stimulatory and inhibitory effects on oxidative stress (OS). We have previously demonstrated that endotoxin (ET)‐induced fall in BP is associated with an increase in nitrite levels in serum, kidney, heart, thoracic aorta (TA) and superior mesenteric artery (SMA), a decrease in MDA levels in the kidney, heart, TA and SMA, and an increase in MPO activity in the heart and TA, but a decrease in the kidney and SMA of rats. We further investigated the effect of NO on the markers of OS in other tissues of the endotoxemic rats. ET‐induced increase in nitrite production was associated with a decrease in GSH levels in the liver, lungs, brain, spleen and femoral artery (FA). MPO activity was increased by ET in the lungs, spleen and FA, but was decreased in the liver and brain. MDA levels were increased by ET in the lungs, brain, spleen and FA, but were decreased in the liver. SOD and catalase activity was decreased in the liver and spleen, but was increased in the lungs, brain and FA. The effects of ET were prevented by an iNOS inhibitor. The data suggest that NO differentially regulates OS depending on the tissue during endotoxemia. This study was supported by Mersin University Research Foundation.