Premium
Nitric Oxide‐Mediated Growth Inhibition of an Endothelialized Tissue Engineered Aortic Valve
Author(s) -
HageraatsBoucher JamiLee,
Boughner Derek R.,
Rogers Kem A.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.903.7
Subject(s) - nitric oxide , chemistry , endothelium , in vitro , matrigel , tissue engineering , scaffold , microbiology and biotechnology , anatomy , biomedical engineering , medicine , biology , biochemistry , organic chemistry
The tissue engineering (TE) of aortic valves may provide an alternative to the use of non‐viable bioprosthetic replacements. Endothelialization of a TE construct is necessary to establish a non‐thrombogenic surface and has been shown to inhibit the proliferation of vascular smooth muscle cells within the construct. Previously it has been shown that nitric oxide (NO) production by endothelial cells may be responsible for this growth inhibition. To test this hypothesis, cells scaffold constructs (CSCs) were produced consisting of radial artery cells seeded onto small intestine submucosa. After one week in culture, an analog inhibitor of nitric oxide synthase, L‐iminoethyl‐L‐orthinine (L‐NIO, 100 μM) was added to either CSCs or endothelialiazed CSCs (ECSCs). Constructs were cultured for an additional week, followed by fixation and tissue thickness analysis by confocal microscopy. ECSC and CSC control groups were cultured for two weeks in the absence of L‐NIO. The presence of an endothelium (ECSCs) significantly inhibited growth of the construct, however, in the presence of L‐NIO, growth of the construct continued in a manner similar to CSCs (75.6μ m vs 55.3μm). Results suggest that the production of NO by the endothelium may be responsible for growth inhibition of the ECSCs providing a valuable tool for controlling TE valve construct thickness in vitro . Funding provided by Heart and Stroke foundation of Ontario.