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Differential Bone Marrow Stem Cell Mobilization by G‐CSF Injection or Arterial Ligation in Baboons
Author(s) -
Shi Qiang,
Wang Xingli,
VandeBerg John L.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.901.6
Subject(s) - ligation , bone marrow , cd34 , stem cell , endothelial stem cell , cd31 , medicine , immunology , chemistry , pathology , biology , andrology , angiogenesis , microbiology and biotechnology , in vitro , biochemistry
In the current study, we investigated whether G‐CSF‐mobilized BMSCs share the same subtype profiles as the ischemia‐induced repair in nonhuman primate baboons. Methods and Results: We subjected 5 baboons to femoral artery ligation and 5 baboons to subcutaneous G‐CSF injection. Blood BMSCs were measured by surface antigens; functional differentiation to endothelial cells was assessed by colony forming capacity, expression of mature endothelial cell antigens and tube‐like formation. The blood CD34+ cells were spiked on day 3 post‐stimulation in both groups. While CD34+ cells released by artery ligation was 2‐fold lower than those by G‐CSF administration, significantly more CD133+/KDR+/CXCR4+/CD31+ cells were detected in baboons with artery ligation. After culture in endothelial growth medium, mononuclear cells from baboons with artery ligation formed more endothelial cell colonies (p < 0.05), formed more capillary‐like tubes (p < 0.05), expressed higher vWF and phagocytosed more Dil‐LDL (p < 0.05). Conclusions: While G‐CSF and artery ligation can mobilize BMSCs capable of differentiating into endothelial cells, BMSCs mobilized by the artery ligation simulating in vivo ischemic attacks have higher potential for vascular differentiation. Our findings suggest that direct bone marrow aspiration may provide the pool of BMSCs capable of transforming into mature cardiovascular cells.