Premium
New role of Human myofibroblasts on neovascularisation during wound healing
Author(s) -
Mayrand Dominique,
Cordier Benoît,
Blanchette Samuel,
LopezVallé Carlos A.,
Roy Michel,
Genest Hervé,
Moulin Véronique
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.901.1
Subject(s) - wound healing , myofibroblast , neovascularization , angiogenesis , pathology , scars , fibrin , context (archaeology) , medicine , cancer research , biology , immunology , fibrosis , paleontology
Myofibroblasts are involved in healing mechanism and on tumor development. In both cases, these processes are carried out in synergy with revascularization ensuring the survival of the tissue. We used a three‐dimensional model to investigate the role of myofibroblasts on neovascularization in a context of wound healing. The angiogenic development was analyzed in a fibrin‐based matrix after 14 days via a co‐culture of human skin microvascular endothelial cells (MVEC) and normal skin wound myofibroblasts (Wmyo) isolated from the same patient. Human skin fibroblasts (Fb) was used as control. In our culture conditions, we observed a significant increase of capillary‐like structure number and length when MVEC were co‐cultured with Wmyo instead of Fb. In the same way, we used myofibroblasts (Hmyo) and microvascular endothelial cells (MVECH) isolated from human hypertrophic scars. A positive effect of Hmyo addition on the angiogenic process was also observed but to a lesser extent than with Wmyo. The MVEC and MVECH had similar responses in the presence of dermal cells. From these results, we hypothesize that myofibroblasts could have a more important role on neovascularization than expected on normal wound healing but also in hypertrophic scarring characterized by a large number of myofibroblasts and an extensive microvascular network. This study was supported by CIHR and a scholarship from FRSQ (VM).