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Plasma membrane cholesterol modulates Campylobacter jejuni‐induced translocation of non‐invasive intestinal bacteria
Author(s) -
Kalischuk Lisa D,
Inglis G. Douglas,
Buret Andre G
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.899.12
Subject(s) - campylobacter jejuni , chromosomal translocation , microbiology and biotechnology , internalization , mesenteric lymph nodes , endocytosis , lipid raft , pathogenesis , biology , bacteria , immunology , signal transduction , biochemistry , immune system , genetics , cell , gene
Bacterial enteritis, caused by pathogens such as Campylobacter jejuni , has been implicated as a factor that exacerbates inflammatory bowel disease (IBD) symptoms. The mechanisms whereby translocation of intestinal bacteria secondary to epithelial barrier dysfunction may contribute to the pathogenesis of IBD, have yet to be elucidated. Aims: The aims of this study were to determine whether C. jejuni induces translocation of non‐invasive intestinal bacteria and examine the mechanisms by which this occurs. Results: Wild‐type C. jejuni 81‐176, but not an invasion‐defective FlaAFlaB mutant, induced translocation of intestinal bacteria to the spleens, livers, and mesenteric lymph nodes of infected mice. In human colonic T84 monolayers, C. jejuni induced internalization and translocation of non‐invasive Escherichia coli HB101 via a transcellular pathway in the absence of increased epithelial permeability to a 3 kDa dextran probe. Translocation was prevented by depletion of plasma membrane cholesterol with MbetaCD/lovastatin, and translocating E. coli co‐localized with lipid raft caveolin. Conclusion: C. jejuni may contribute to the pathogenesis of IBD by inducing the internalization and translocation of non‐invasive intestinal bacteria via a mechanism consistent with lipid raft‐mediated endocytosis. Research supported by NSERC and the Crohn's and Colitis Foundation of Canada.