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The effect of RNA inteference‐medicated inhibition of VASP expression on migration of MCF‐7 cells
Author(s) -
zhou xuan,
Wei Lei,
Chen Huaqi,
Wang Jing,
Wei Yun
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.898.20
Subject(s) - small interfering rna , gene knockdown , cell migration , microbiology and biotechnology , rna interference , gene silencing , transfection , lipofectamine , rac1 , chemistry , lamellipodium , actin cytoskeleton , mcf 7 , cell , cytoskeleton , biology , rna , cancer cell , signal transduction , gene , biochemistry , cancer , human breast , genetics , vector (molecular biology) , recombinant dna
Cell migration is critical to many pathophysiological processes, such as tumor invasion and metastasis. It is a complicated process that is regulated by cell‐matrix adhesions and actin cytoskeleton. We report here that Vasodilator‐stimulated phosphoprotein (VASP), which has complex effects on cytoskeletal organization and cell motility. Small interfering RNAs(siRNAs) mediate RNA interference(RNAi), providing a powerful new tool for gene silencing. Herein, we explore the effect of VASP‐siRNAs on migration of MCF‐7 cells. Three pairs of VASP‐siRNAs (942, 1002, 1024) were designed, which were transfected into MCF‐7 cells via Lipofectamine 2000. To evaluate the inhibition activity of VASP‐siRNAs, VASP expression were examined with RT‐PCR and Western blotting. In MCF‐7 cells, we observed that VASP‐942 siRNA could effectively inhibit VASP expression at mRNA and protein level with a concomitant decrease of migration significantly by a wound‐healing assay. Meanwhile, the activation of Rac1 pathway by fMLP could promote the migration of MCF‐7 cells via elevating the VASP expression level. Thus, our study showed that the knockdown of VASP contributes to the reduction of migration in MCF‐7 cells. It provided that the important role of VASP in breast tumour metastasis might be regulated through Rac1 pathway. (This work was supported by National Natural Sciences Foundation of China under Grant No.30570751)