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Pathological Changes In Melanoma Induced by Multiple Nanosecond Pulsed Electric Fields Treatments And Possible Mechanism Analysis
Author(s) -
chen xinhua,
Swanson Robbert James,
Schoenbach Karl H,
Zheng Shusen
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.898.1
Subject(s) - melanoma , apoptosis , tunel assay , immunohistochemistry , pathological , chemistry , cancer research , nanosecond , histone , intracellular , medicine , pathology , biochemistry , dna , laser , physics , optics
Objective : Nanosecond pulsed electric fields (nsPEFs) can affect the intracellular structures of cells in vitro . This study is to reveal the pathological characteristics of B16‐F10 melanoma on SKH‐1 mice exposed to nsPEFs and analysis of the possible underlying mechanism. Methods: Melanoma model was set up by injecting B16‐F10 on 120 female SKH‐1 mice. After the multiple treatments nsPEFs ( intensity as 100 kV/cm and duration as 300 nanosecond ), morphologic changes of melanoma was detected and Caspase‐3,6,7 and histone H2AX were also Results: After multiple nsPEFs, the melanoma blood supply was blocked and melanoma shrink 90% compared to the control group. Histological exams revealed the features of increased metabolic activity and apoptosis body in the treated melanomas. Moreover, TUNEL showed an increase in the apoptotic activity in treated melanoma. These alterations were associated with caspase3,6,7 and histone H2AX increased significantly after pulse as revealed by immunohistochemistry. Conclusion: nsPEFs has potential applications in cancer therapy,