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Induction of experimental colitis by dextran sulphate sodium in a piglet model of irritable bowel syndrome
Author(s) -
Lackeyram Dale,
Kim Connie,
Young Denise,
Archbold Tania,
Yang Chengbo,
Mine Yoshinori,
Fan Ming
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.896.8
Subject(s) - mannitol , saline , irritable bowel syndrome , medicine , bolus (digestion) , gastroenterology , colitis , chemistry , biochemistry
This study was conducted for chemical induction of intestinal inflammation with dextran sodium sulphate (DSS) in piglets. A total of 11 Yorkshire piglets of 5 d of age, with an average body weight (BW) of 3 kg, were fitted with intra‐gastric catheters and divided into a control (CON, n=6) and treatment groups (DSS, n=5). Both groups were infused with equal volumes of either saline or 1.25 g of DSS/kg BW.d in saline, respectively, for 10 d. Histological grading of the colon indicated that DSS animals had severe, transmural inflammation. ELISA analysis indicated that DSS‐treated piglets had higher ( P <0.05) levels of the pro‐inflammatory cytokines TNF‐α and IL6 in the colon (DSS, 476.64±62.91 vs. CON, 83.66±22.52 and DSS, 189.72±9.93 vs. CON, 64.91±5.84, pg/g tissue), respectively. Quantitative real time RT‐PCR confirmed higher ( P <0.05) expression levels of TNF‐α mRNA in DSS‐treated pigs (DSS, 1.21±0.51 vs. CON, 0.011±0.002, arbitrary units) while the anti‐inflammatory cytokine IL10 was undetectable (DSS, ND vs. CON, 0.025±0.005, arbitrary units) in the colon of DSS‐treated piglets. Gut D‐mannitol permeability into blood circulation subsequent to an intra‐gastric bolus infusion of D‐mannitol (0.6 g/kg BW) after 75 min was higher ( P <0.05) in the DSS group (DSS, y = 1.691x, r 2 = 0.97 vs. CON, y = 0.463x, r 2 = 0.93). We conclude that the dosage of 1.25 g DSS/kg BW.d is suitable to induce experimental colitis and IBS pathology in piglets. Supported by OMAFRA of Canada.