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Profiling Histidine Dipeptides in Plasma and Urine After Ingesting Beef, Chicken or Chicken Broth in Humans
Author(s) -
Yeum KyungJin,
Aldini Giancarlo,
Rasmussen Helen,
Carini Marina,
Russell Robert M
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.890.3
Subject(s) - carnosine , anserine , chemistry , histidine , urine , ingestion , food science , chromatography , biochemistry , amino acid
Reactive carbonyl species (RCS), oxidation products of polyunsaturated fatty acids, protein & sugars, play a role in the etiology of certain chronic diseases. Our previous studies revealed that histidine‐dipeptides such as carnosine and anserine detoxify cytotoxic carbonyls such as 4‐hydroxy‐ trans ‐2‐nonenal & acrolein by forming unreactive adducts. In the current study, pharmacokinetics of histidine‐dipeptides has been determined after ingesting histidine‐dipeptides rich foods. Healthy women (n=4) went through 3 phases of one dose of either 150g beef (B), 150g chicken (C), or chicken broth (CB) from 150g chicken with >2 wks of wash out period between each phase. Blood samples were collected 0‐300 min and urine samples before & after (up to 7 hrs) ingesting the food and analyzed for histidine dipeptides using LC‐ESI‐MS/MS. Only a negligible amount of carnosine was detected in plasma after ingesting B, C or CB. However, plasma anserine concentration was significantly increased (p<0.05) to 2.72 ± 1.08 μmol/L at 100 min and 0.78 ± 0.18 μmol/L at 60 min after ingesting C and CB, respectively. Urinary carnosine and anserine were increased 13.8 & 13.7 fold after ingesting B and 14.8 & 243 fold after CB ingestion, respectively. Thus, dietary histidine dipeptides, which are rapidly hydrolyzed by carnosinase in plasma & excreted in minor fraction in urine, may act as RCS sequestering agents. (Supported by USDA 58‐1950‐7‐707)