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Effect of Date Pits on Oxidative Damage and Antioxidant Defense Systems in Rat Tissues
Author(s) -
Habib Hosam H.,
Ibrahim Wissam H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.890.26
Subject(s) - malondialdehyde , lipid peroxidation , antioxidant , ascorbic acid , glutathione peroxidase , catalase , glutathione , chemistry , vitamin e , in vivo , oxidative stress , medicine , biochemistry , endocrinology , vitamin c , biology , enzyme , food science , microbiology and biotechnology
The purpose of this study was to determine the effect of date pits on oxidative damage and antioxidant status in vivo. Male Wistar rats (six weeks old) were fed a basal diet with either 0%, 7% or 14 % date pits for 30 days. All three diets were isonitrogenous and isocaloric. At the end of the feeding period, rats were killed and liver homogenate and serum were analyzed for the levels of the lipid peroxidation product malondialdehyde (MDA). Additionally, the levels of the antioxidants glutathione, ascorbic acid, and vitamin E, and the antioxidant enzymes glutathione peroxidase, and catalase were analyzed in the liver. Compared with the control group, rats fed either 7% or 14% date pits had significantly (P < 0.05) lower levels of serum MDA or liver MDA. There was no significant difference in serum MDA or liver MDA between rats fed 7% and those fed 14% dates pits. The levels of vitamin E, GSH, and ascorbic acid and the activities of glutathione peroxidase and catalase were not significantly altered by date pits. The results obtained provide direct experimental evidence of the antioxidant effect of date pits in vivo.