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HEPATOPROTECTIVE EFFECT OF SILICA HYDRIDE ON CARBON TETRACHLORIDE‐INDUCED HEPATIC INJURY IN RATS
Author(s) -
Mendieta Jessica Elena,
Correa José,
Trujillo José,
Rosales Martha
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.890.25
Subject(s) - chemistry , antioxidant , carbon tetrachloride , glutathione , reactive oxygen species , in vivo , lipid peroxidation , pharmacology , oxidative stress , glutathione reductase , biochemistry , scavenger , glutathione peroxidase , enzyme , organic chemistry , biology , microbiology and biotechnology
Reactive oxygen species (ROS) have been proven to cause numerous cellular anomalies, which cause oxidative stress. Taking into account that the most endogenous and exogenous antioxidants are not enough effective as reducing agents and as ROS selective scavengers, an anionic hydride organosiloxane, commonly named silica hydride (SiH − ), was previously evaluated in vitro. However, none research had been done to prove its antioxidant and protective effects in vivo. For this reason, this work was aimed to demonstrate it suggesting its possible mechanism of action. The results of the present study demonstrate that the protective effect of SiH − is dose‐dependent. Pre‐treatment of Sprague‐Dawley rats with dosages of 180 and 200 mg/kg showed the better protection against CCl 4 ‐induced hepatotoxicity. Lipid peroxidation and cytochrome P‐450 activity significantly decreased in relation to that of controls. In contrast, glutathione content, glutathione peroxidase and glutathione reductase activities significantly increased. The lower serum levels of alanine and aspartate amino transferases were restored. Histopathological studies confirm the protective effect of SiH − . In conclusion, this model in vivo and the different assays confirm that the mechanism of hepatoprotective action of SiH − may be due to its antioxidant and radical scavenger activities.